Portal de Periódicos da CAPES

Large-Scale Production of Porcine Mature Interleukin-18 (IL-18) in Silkworms Using a Hybrid Baculovirus Expression System.

2003; Japanese Society of Veterinary Science; Volume: 65; Issue: 2 Linguagem: Inglês

10.1292/jvms.65.219

1347-7439

Yoshihiro Muneta, Hong Kun Zhao, Shigeki Inumaru, Yasuyuki Mori,

Virus-based gene therapy research

In this report, a hybrid baculovirus expression system, which means a hybrid virus of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (IL-18) in silkworms. Two recombinant hybrid baculoviruses containing cDNA of the porcine precursor IL-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. After the co-infection of the two viruses, porcine mature IL-18 was efficiently produced in the haemolymph. The concentration of IL-18 in the haemolymph was 80-100 μg/ml, as determined by porcine IL-18 specific ELISA. This yield was twenty-times more than that of the insect cell expression system described previously. The porcine mature IL-18 produced by the silkworms strongly induced interferon-γ (IFN-γ) production from porcine PBMC. An insect factory system for the large-scale production of useful cytokines for livestock animals will be available in the near future.