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Determinação de carbaril utilizando testes ELISA (Enzyme-linked immunosorbent assay) e CLAE com detecção por arranjo de diodos

2000; Brazilian Chemical Society; Volume: 23; Issue: 4 Linguagem: Inglês

10.1590/s0100-40422000000400007

ISSN

1678-7064

Autores

Ilda Antonieta Salata Toscano, Maria Lúcia Ribeiro, Júlio César Rocha, Gilvanda Silva Nunes, ‪Damià Barceló,

Tópico(s)

Dye analysis and toxicity

Resumo

DETERMINATION OF CARBARYL BY ELISA (ENZYME-LINKED IMMUNOSORBENT AS-SAY) AND HPLC WITH PHOTODIODE ARRAY DETECTION. ELISAs have been appliedto pesticide residue analysis due to their high sensitivity and selectivity.However, some ELISAs performance may be affected by matrix components.In this work, ELISA for carbaryl in water samples was checked for interference by naturally occurring fulvic acids.The results suggested that the high fulvic acid concentration (≥30 mg L -1 ) and acidic pH conditions (pH 4.0) interfere with the signal detection decreasing the method sensitivity.A dilution of the samples and adjust to pH 8.0 are appropriate to minimize the matrix interferences in the ELISA method.Good correlation between ELISA and HPLC-DAD results was observed.

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