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Dibutyryl cyclic amp affects hyaluronate synthesis and macromolecular organization in normal adult articular cartilage in vitro

1980; Elsevier BV; Volume: 631; Issue: 2 Linguagem: Inglês

10.1016/0304-4165(80)90301-3

1872-8006

Michael T. Stack, Kenneth D. Brandt,

Cell Adhesion Molecules Research

When normal adult dog articular cartilage was cultured in the presence of dibutyryl cyclic AMP a higher proportion than normal of newly synthesized 35S-labeled glycosaminoglycans was released from the tissue into the culture medium, although their net synthesis was not affected. In conjunction with this release of sulfated glycosaminoglycans, 24 times more [3H]glucosamine-labeled hyaluronic acid was released from the cartilage into the medium, and net hyaluronate synthesis was enhanced 3-fold. Virtually all of the newly synthesized hyaluronic acid in the medium was associated with proteoglycans. The proteoglycans in the medium of the dibutyryl cyclic AMP treated cultures were normal in hydrodynamic size and interacted normally with hyluronic acid to form large aggregates. These results suggest that the increase in hylarunate synthesis caused by dibutyryl cyclic AMP may have destabilized the interaction of protoeglycans with the collagen meshwork of the cartilage. The changes seen in normal adult articular cartilage after incubation with dibutyryl cyclic AMP, therefore, are similar to those whic are observed in cartilage of osteoarthritic joints.