A response to ‘Cerebral and extracerebral release of protein S100B in cardiac surgical patients’, Snyder‐Ramos SA, Gruhlke T, Bauer H, Bauer M, Luntz AP, Motsch J, Martin E, Vahl CF, Missler U, Wiesmann M and Bottiger BW, Anaesthesia 2004; 59: 344–9
2004; Wiley; Volume: 59; Issue: 11 Linguagem: Inglês
10.1111/j.1365-2044.2004.04003.x
ISSN1365-2044
Autores Tópico(s)Sepsis Diagnosis and Treatment
ResumoI read with interest this article describing the artifactual nature of the early S100β increases after CPB. In a recent work, we have shown that these exorbitant levels do not derive from cardiac (or other non-CNS) tissue, but rather reflect a cross-reactivity between the antibodies employed for the study [1]. This is an important issue, since it also addresses the question of why putative increases in peripheral S100β occur in the absence of neuronal or cerebrovascular damage. Since the offending antigens belong to a family of high molecular weight protein, serum filtration prevents false reading of peripheral levels of S100β. We have shown that during surgical procedures identical to those described here, S100β levels increases are consistent with early damage to the cerebral vasculature. This was evident only when non-specific binding to non-S100β protein was taken into account and subtracted from the readout.
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