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Estimating Human Inbreeding Coefficients: Comparison of Genealogical and Marker Heterozygosity Approaches

2006; Wiley; Volume: 70; Issue: 5 Linguagem: Inglês

10.1111/j.1469-1809.2006.00263.x

1469-1809

A D Carothers, Igor Rudan, Ivana Kolčić, Ozren Polašek, Caroline Hayward, A F Wright, Harry Campbell, Peter Teague, N D Hastie, J. L. Weber,

Genetic Associations and Epidemiology

We have used genealogies and genomic polymorphisms to estimate individual inbreeding coefficients (F) in 50 subjects with an expected range (based on recent genealogies) of F from 0.0 to 0.0625. The estimates were based on two approaches, using genotypes respectively from 410 microsatellite markers (410-STR panel) and from 10,000 SNPs (10K-SNP panel). The latter was performed in a sub-sample of 15 individuals. We concluded that for both marker panels measures of inbreeding based on the excess of homozygosity over Hardy-Weinberg expectation were not closely correlated with 4-5 generation genealogical F-values. For the 10K-SNP panel we found two alternative measures which correlated more closely with F, based respectively on standard errors and on paired homozygosity of nearby SNPs over distances of 2-4 cM. We propose an empirical method for estimating standard errors and hence individual F-values, based on the variation between individual autosomes. This method could provide useful estimates of average F-values for groups of individuals in population-based studies of the effects of inbreeding/homozygosity on quantitative traits.