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Methanogenic Bacterium Gö1: An Acetoclastic Methanogen that is Closely Related to Methanosarcina frisia

1992; Elsevier BV; Volume: 15; Issue: 4 Linguagem: Inglês

10.1016/s0723-2020(11)80118-9

1618-0984

Rik I.L. Eggen, Ans C. M. Geerling, Piet W. J. de Groot, Wolfgang Ludwig, Willem M. de Vos,

Genomics and Phylogenetic Studies

Summary Methanogenic bacterium Go1, an acetoclastic archaeon, appeared to contain three putative 16S rRNA copies. One of these was cloned and further characterized. Nucleotide sequence analysis of the 16S rRNA gene and its flanking regions revealed an organization similar to that of other methanogens, with rRNA's in the order 16S–23S rRNA and a putative tRNA ala in the 16S–23S intergenic region. The 16S rRNA gene is flanked by sequences that can adopt a rRNA maturation stem-loop structure. A phylogenetic relationship with other methanogens was calculated, based on the nucleotide sequence of the 16S rRNA gene. This indicated that the methanogenic bacterium Go1 was closely related to Methanosarcina frisia (99.6% sequence identity). Hence we propose to rename the methanogenic bacterium Go1 into Methanosarcina frisia Go1.