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Liposomes act as stronger sub-unit vaccine adjuvants when compared to microspheres

2008; Taylor & Francis; Volume: 16; Issue: 7-8 Linguagem: Inglês

10.1080/10611860802228558

1061-186X

Daniel Kirby, Ida Rosenkrands, Else Marie Agger, Peter Andersen, Allan G.A. Coombes, Yvonne Perrie,

RNA Interference and Gene Delivery

The ability of liposomes and microspheres to enhance the efficacy of a sub-unit antigen was investigated. Microspheres were optimised by testing a range of surfactants employed in the external aqueous phase of a water-in-oil-in-water (w/o/w) double emulsion solvent evaporation process for the preparation of microspheres--composed of poly(D,L-lactide-co-glycolide) and the immunological adjuvant dimethyl dioctadecyl ammonium bromide (DDA)--and then investigated with regard to the physico-chemical and immunological characteristics of the particles produced. The results demonstrate that this parameter can affect the physico-chemical characteristics of these systems and subsequently, has a substantial bearing on the level of immune response achieved, both humoral and cell mediated, when employed for the delivery of the sub-unit tuberculosis vaccine antigen Ag85B-ESAT-6. Moreover, the microsphere preparations investigated failed to initiate immune responses at the levels achieved with an adjuvant DDA-based liposome formulation (DDA-TDB), further substantiating the superior ability of liposomes as vaccine delivery systems.