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Nonlinearity in protein assays by the Coomassie blue dye-binding method

1989; Elsevier BV; Volume: 179; Issue: 1 Linguagem: Inglês

10.1016/0003-2697(89)90225-x

1096-0309

A. Splittgerber, Julie L. Sohl,

Mass Spectrometry Techniques and Applications

The Coomassie brilliant blue (CBB) method for protein determination takes advantage of the fact that a low-pH red form of CBB reverts to a blue form when CBB binds to protein. The increase in absorbance at 595 nm of a protein-dye mixture compared to a blank containing only the dye reagent has been used to estimate the total amount of protein present in the sample mixture. A disadvantage of this method of protein determination is that the assay plot of absorbance at 595 nm versus total protein is not linear.