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Determination of enantiomers of bupivacaine in serum using an on-line coupled three column liquid chromatographic system

1993; Elsevier BV; Volume: 11; Issue: 3 Linguagem: Inglês

10.1016/0731-7085(93)80201-b

1873-264X

Jan J. Butter, J.C. Kraak, H. Poppe,

Anesthesia and Sedative Agents

An on-line coupled HPLC system is described for the determination of the enantiomers of bupivacaine in serum. The method involves three steps: (i) pre-concentration and clean-up; (ii) the determination of the racemates on a reversed-phase column; and (iii) the separation of the racemates, heart-cut from the reversed-phase column, on an alpha-glycoprotein column. The method is suitable to determine the enantiomers in serum down to 0.1 micrograms ml-1 and can be fully automated. The bupivacaine time course of patients will be shown.