Light-Controlled Molecular Shuttles Made from Motor Proteins Carrying Cargo on Engineered Surfaces
2001; American Chemical Society; Volume: 1; Issue: 5 Linguagem: Inglês
10.1021/nl015521e
ISSN1530-6992
AutoresHenry Hess, John Clemmens, Dong Qin, Jonathon Howard, Viola Vogel,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoMolecular shuttles have been built from motor proteins capable of moving cargo along engineered paths. We illustrate alternative methods of controlling the direction of motion of microtubules on engineered kinesin tracks, how to load cargo covalently to microtubules, and how to exploit UV-induced release of caged ATP combined with enzymatic ATP degradation by hexokinase to turn the shuttles on and off sequentially. These are the first steps in the development of a tool kit to utilize molecular motors for the construction of nanoscale assembly lines.
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