Stimulated astrocytes release high-mobility group 1 protein, an inducer of LAN-5 neuroblastoma cell differentiation
1997; Elsevier BV; Volume: 82; Issue: 4 Linguagem: Inglês
10.1016/s0306-4522(97)00352-7
ISSN1873-7544
AutoresMario Passalacqua, Mauro Patrone, Giovanni B. Picotti, Marcela Del Río, Bianca Sparatore, E. Melloni, S. Pontremoli,
Tópico(s)Cell Adhesion Molecules Research
ResumoStimulated astrocytes specifically release large amounts of high-mobility group 1 protein into the extracellular medium of the rat. The identity of the released protein has been established on the basis of its biological activity on murine erythroleukaemia cells and by its immunoreactivity against a specific monoclonal antibody. High-mobility group 1 protein also plays an essential role in differentiation of LAN-5 neuroblastoma cells which, following stimulation with retinoic acid, express high-mobility group 1 protein on to the external surface of the plasma membrane. In retinoic acid-induced LAN-5 cells, high-mobility group 1 protein is not secreted but is accumulated in a membrane-bound form, particularly at the level of neurite outgrowths. These cells can also be induced to differentiate by high-mobility group 1 protein coated on the surface of the cell culture vessels. The specific function of the protein in this process is indicated by inhibition of cell differentiation by an anti-high-mobility group 1 protein antibody. The data are consistent with a role of high-mobility group 1 protein in promoting cell–cell interactions and in the development of nerve tissues.
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