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Recovery of bacterial spores dried on aluminium strips

1996; Wiley; Volume: 80; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2672.1996.tb03202.x

2056-5232

E Sugimoto, Andrea Raasch, Robson M. Ehioba,

Bacillus and Francisella bacterial research

Bacterial spores dried on aluminium strips are used in microbiological validation of packaging and processing systems. Vortex agitation and sonication in Butterfield's buffer, 70% ethanol or 0·1% Tween 80 were evaluated for ease of recovery of bacillus spores dried on aluminium strips to compare the concentration of dried spores to dilutions used to inoculate such strips. The highest recovery for Bacillus subtilis var. globigii spores was observed with sonication in 70% ethanol with average recovery close to the initial inoculum. The highest recovery for B. stearothermophilus spores was with sonication in Butterfield's buffer, averaging 0·8 log less recovery than the initial inoculum. Bacillus subtilis var. globigii spores were recovered from strips in greater numbers than B. stearothermophilus spores for all treatment medium combinations. Scanning electron microscopy revealed unrecovered spores adhering to strips after treatment. Recovery of B. subtilis var. globigii spores decreased with time over the 4 week storage period.