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Dissociation constants of water-insoluble carboxylic acids by13C-NMR. pK as of mesobiliverdin-XIIIα and mesobilirubin-XIIIα

1996; Birkhäuser; Volume: 52; Issue: 6 Linguagem: Inglês

10.1007/bf01969747

0014-4754

David A. Lightner, Dave Holmes, Antony F. McDonagh,

Hemoglobin structure and function

High-field 13C-NMR of 13C-enriched compounds in dilute aqueous d6-Me2-SO solutions provides a simple, accurate method for measuring pK(a)s of sparingly soluble carboxylic acids. Using this method, we found the pK(a)s of mesobilirubin-XIII alpha to be 4.2 and 4.9, much lower values than reported recently for bilirubin, and of mesobiliverdin-XIII alpha to be 3.9 and 5.3.