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Advances in in-situ product recovery (ISPR) in whole cell biotechnology during the last decade

2014; Elsevier BV; Volume: 32; Issue: 7 Linguagem: Inglês

10.1016/j.biotechadv.2014.07.003

1873-1899

Wouter Van Hecke, Guneet Kaur, Heleen De Wever,

Enzyme Catalysis and Immobilization

The review presents the state-of-the-art in the applications of in-situ product recovery (ISPR) in whole-cell biotechnology over the last 10 years. It summarizes various ISPR-integrated fermentation processes for the production of a wide spectrum of bio-based products. A critical assessment of the performance of various ISPR concepts with respect to the degree of product enrichment, improved productivity, reduced process flows and increased yields is provided. Requirements to allow a successful industrial implementation of ISPR are also discussed. Finally, supporting technologies such as online monitoring, mathematical modeling and use of recombinant microorganisms with ISPR are presented.