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Thin Layer Chromatography/Fluorescence Detection of 3,4‐Methylenedioxy‐Methamphetamine and Related Compounds

2008; Wiley; Volume: 53; Issue: 6 Linguagem: Inglês

10.1111/j.1556-4029.2008.00870.x

1556-4029

Noriyuki Kato, Susumu Fujita, Hikoto Ohta, Makoto Fukuba, Akira Toriba, Kazuichi Hayakawa,

Neurotransmitter Receptor Influence on Behavior

A rapid and sensitive method for the detection of six methylenedioxylatedphenethylamines, 3,4-methylenedioxymethamphetamine (MDMA); 3,4-methylenedioxyamphetamine; 3,4-methylenedioxyethylamphetamine; N-methyl-1-(3,4-methylenedioxyphenyl)-2-butamine; N-methyl-1-(3,4-methylenedioxyphenyl)-3-butamine; and 3,4-methylenedioxydimethylamphetamine, by thin-layer chromatography with fluorescence detection is proposed. These compounds form fluorophores on the developing plate following spraying with a reagent consisting of sodium hypochlorite, potassium hexacyanoferrate (III), and sodium hydroxide, and heating for 3 min at 100 degrees C. Blue fluorescent spots were observed under ultraviolet light in a wavelength range of 250-400 nm. The detection limits for MDMA and the above related compounds were 50 ng. The proposed method was effectively applied to the detection of MDMA in urine samples.