Monitoring nitric oxide (NO) in rat locus coeruleus
1997; Lippincott Williams & Wilkins; Volume: 8; Issue: 6 Linguagem: Inglês
10.1097/00001756-199704140-00001
ISSN1473-558X
AutoresChristophe Desvignes, F. Robert, Cédric Vachette, Guy Chouvet, Raymond Cespuglio, Bernard Renaud, Laura Lambás‐Señas,
Tópico(s)Receptor Mechanisms and Signaling
ResumoA porphyrinic microsensor combined with in vivo voltammetry was used to monitor extracellular nitric oxide (NO) in the locus coeruleus (LC) of anaesthetized rats. Administration of Nω-nitro-L-arginine p-nitroanilide (100 mg/kg, i.p) or 7-nitro indazole (30 mg/kg, i.p.), which both inhibit preferentially neuronal NO synthase (NOS), induced a marked decrease in the NO oxidation peak height. On the other hand, Nw-nitro-L-arginine methyl ester (L-NAME) (200 mg/kg, i.p.), a less selective NOS inhibitor, failed to decrease the NO signal. Moreover, intra LC administration of NMDA, known to activate LC noradrenergic neurones, increased the NO signal. This study demonstrates the usefulness of in vivo voltammetry to monitor basal levels of NO and their changes in the LC. Differential effects of NOS inhibitors show that their central activity need to be assessed through in situ measurement of NO before using these inhibitors as neuropharmacological tools.
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