Brain and liver mitochondria isolated from diabeticGoto-Kakizaki rats show different susceptibility to induced oxidative stress
2001; Wiley; Volume: 17; Issue: 3 Linguagem: Inglês
10.1002/dmrr.200
ISSN1520-7560
AutoresMaria S. Santos, Dario L. Santos, Carlos M. Palmeira, Raquel Sei�a, António J. Moreno, Catarina R. Oliveira,
Tópico(s)Biochemical effects in animals
ResumoDiabetes/Metabolism Research and ReviewsVolume 17, Issue 3 p. 223-230 Research Article Brain and liver mitochondria isolated from diabeticGoto-Kakizaki rats show different susceptibility to induced oxidative stress Maria S. Santos, Maria S. Santos Center for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, PortugalSearch for more papers by this authorDario L. Santos, Dario L. Santos University of Trás os Montes e Alto Douro, Vila Real, PortugalSearch for more papers by this authorCarlos M. Palmeira, Corresponding Author Carlos M. Palmeira [email protected] Center for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, PortugalCenter for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.Search for more papers by this authorRaquel Seiça, Raquel Seiça Faculty of Medicine, University of Coimbra, Coimbra, PortugalSearch for more papers by this authorAntónio J. Moreno, António J. Moreno Center for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, PortugalSearch for more papers by this authorCatarina R. Oliveira, Catarina R. Oliveira Faculty of Medicine, University of Coimbra, Coimbra, PortugalSearch for more papers by this author Maria S. Santos, Maria S. Santos Center for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, PortugalSearch for more papers by this authorDario L. Santos, Dario L. Santos University of Trás os Montes e Alto Douro, Vila Real, PortugalSearch for more papers by this authorCarlos M. Palmeira, Corresponding Author Carlos M. Palmeira [email protected] Center for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, PortugalCenter for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, 3004-517 Coimbra, Portugal.Search for more papers by this authorRaquel Seiça, Raquel Seiça Faculty of Medicine, University of Coimbra, Coimbra, PortugalSearch for more papers by this authorAntónio J. Moreno, António J. Moreno Center for Neurosciences and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, Coimbra, PortugalSearch for more papers by this authorCatarina R. Oliveira, Catarina R. Oliveira Faculty of Medicine, University of Coimbra, Coimbra, PortugalSearch for more papers by this author First published: 18 June 2001 https://doi.org/10.1002/dmrr.200Citations: 56Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Background Increased oxidative stress and changes in antioxidant capacity observed in both clinical and experimental diabetes mellitus have been implicated in the etiology of chronic diabetic complications. Many authors have shown that hyperglycemia leads to an increase in lipid peroxidation in diabetic patients and animals reflecting a rise in reactive oxygen species production. The aim of the study was to compare the susceptibility of mitochondria from brain and liver of Goto-Kakizaki (12-month-old diabetic) rats (GK rats), a model of non-insulin dependent diabetes mellitus, to oxidative stress and antioxidant defenses. Methods Brain and liver mitochondrial preparations were obtained by differential centrifugation. Oxidative damage injury was induced in vitro by the oxidant pair ADP/Fe2+ and the extent of membrane oxidation was assessed by oxygen consumption, malondialdehyde (MDA) and thiobarbituric acid reactive substances (TBARS) formation. Coenzyme Q and α-tocopherol contents were measured by high-performance liquid chromatography (HPLC). Results Brain mitochondria isolated from 12-month-old control rats displayed a higher susceptibility to lipid peroxidation, as assessed by oxygen consumption and formation of MDA and TBARS, compared to liver mitochondria. In GK rats, mitochondria isolated from brain were more susceptible to invitro oxidative damage than brain mitochondria from normal rats. In contrast, liver mitochondria from diabetic rats were less susceptible to oxidative damage than mitochondria from normal rats. This decreased susceptibility was inversely related to their α-tocopherol and coenzyme Q (CoQ) content. Conclusions The present results indicate that the diabetic state can result in an elevation of both α-tocopherol and CoQ content in liver, which may be involved in the elimination of mitochondrially generated reactive oxygen species. The difference in the antioxidant defense mechanisms in the brain and liver mitochondrial preparations of moderately hyperglycemic diabetic GK rats may correspond to a different adaptive response of the cells to the increased oxidative damage in diabetes. Copyright © 2001 John Wiley & Sons, Ltd. Citing Literature Volume17, Issue3May/June 2001Pages 223-230 RelatedInformation
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