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Transcription termination and RNA processin in the 3‘-end spacer of mouse ribosomalo RNA genes

1987; Oxford University Press; Volume: 15; Issue: 5 Linguagem: Inglês

10.1093/nar/15.5.2043

1362-4962

Takeshi Miwa, Ryo Kominami, Hiroshi Yoshikura, Kazuko Sudo, Masami Muramatsu,

RNA modifications and cancer

The 3' termini of ribosomal RNA precursors from mouse FM3A cultured cells are mapped to eight sites within 625 bp downstream from the 3' terminus of 28 S rRNA. Three additional sites are mapped in liver RNA from C3H/He strain mice. Two of them, the sites at 570 bp and 625 bp are assumed to be termination sites in vivo, because they correspond to in vitro termination sites of RNA polymerase I, and 45 S RNAs having these 3' termini decay with kinetics distinct from others. The amount of 45 S RNA having the 3' terminus at other sites is variable among several mouse strains, despite their having the same DNA sequence in these regions. The ability to produce 3' termini in these sites seems to follow Mendel's law of inheritance. Therefore, we postulate that these nine sites are RNA processing sites which are controlled genetically.