Development of a cytochrome c-based screen-printed biosensor for the determination of the antioxidant capacity of orange juices
2009; Elsevier BV; Volume: 76; Issue: 1-2 Linguagem: Inglês
10.1016/j.bioelechem.2009.04.004
ISSN1878-562X
AutoresMontserrat Cortina‐Puig, Xavier Muñoz‐Berbel, Régis Rouillon, Carole Calas‐Blanchard, Jean‐Louis Marty,
Tópico(s)Analytical Chemistry and Sensors
ResumoThis paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices.
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