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BIBTEX RIS

Use of lymphocyte cultures for BrdU replication banding patterns in anuran species (Amphibia)

1998; Brazilian Society of Genetics; Volume: 21; Issue: 4 Linguagem: Inglês

10.1590/s1415-47571998000400011

1678-4685

Sanae Kasahara, Ana Paula Zampieri Silva, Simone Gruber,

Plant tissue culture and regeneration

We describe the standardization of lymphocyte culture procedures in order to improve cytological preparations of anuran species. This methodology permits the use of 5-bromodeoxyuridine (BrdU) treatment to obtain replication banding patterns in the chromosomes of these species.