Biomagnetic Glasses: Preparation, Characterization, and Biosensor Applications
2009; American Chemical Society; Volume: 26; Issue: 6 Linguagem: Inglês
10.1021/la903422q
ISSN1520-5827
AutoresYu‐Ho Won, Ho Seong Jang, Seung Min Kim, Eric A. Stach, Mallikarjunarao Ganesana, Silvana Andreescu, Lia Stanciu,
Tópico(s)Electrochemical Analysis and Applications
ResumoIn this work, a novel avenue to create a generic approach for the fabrication of biofunctional materials with magnetic capabilities to be used in the design of highly stable, magnetically separable enzyme-based systems was explored. As a model system, immobilization of acetylcholinesterase (AChE) was investigated using biomagnetic glasses composed of a magnetic core with a size tunable porous silica shell. The efficiency of the immobilization was determined by analyzing the biosensing capability of these biomagnetic glasses for the detection of the organophosphorous pesticide paraoxon. Screen printed electrodes with the AChE-biomagnetic glasses showed higher current response and stability than for the free enzyme. The detection limit of the paraoxon biosensor was in the nanomolar range.
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