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Kinetics and metabolic versatility of highly tolerant phenol degrading Alcaligenes strain TW1

2009; Elsevier BV; Volume: 173; Issue: 1-3 Linguagem: Inglês

10.1016/j.jhazmat.2009.09.006

1873-3336

Tamer Essam, Magdy A. Amin, Ossama El Tayeb, Bo Mattìasson, Benoı̂t Guieysse,

Microbial Community Ecology and Physiology

A bacterium that could completely metabolize phenol in batch culture supplied with up to 1200 mg phenol l−1 at room temperature (25 °C) was isolated from the activated sludge of the industrial wastewater treatment plant of a Coke company (Cairo, Egypt). Morphological and physiological characterization showed strain TW1 was a motile, strictly aerobic, gram negative and short-rod occurring singly or in clusters. Partial 16S rRNA gene sequence analysis revealed strain TW1 belonged to the beta group of Proteobacteria, showing 100% identity to Alcaligenes SCTI. Strain TW1 aerobically grew on a number of monocyclic aromatic compounds (hydroquinone, catechol and o-cresol) as well as polycyclic aromatic compounds (pyrene, phenanthrene and naphthalene). The growth of Alcaligenes TW1 on phenol as sole carbon and energy source (25 °C) was well described by the Haldane kinetics model with a maximal specific growth rate of 0.58 h−1, a half-saturation constant of 10 mg l−1, and a substrate inhibition constant of 152–550 mg l−1. The biomass yield coefficient ranged from 0.55 to 0.64 mg dry cell mass/mg phenol. Due to its high tolerance to phenol and high metabolic versatility, Alcaligenes sp. TW1 is considered an excellent candidate for the biotreatment of high strength phenol-laden industrial wastewaters.