Artigo Acesso aberto Revisado por pares

Characterisation of the diol dehydratase pdu operon of Lactobacillus collinoides

2002; Oxford University Press; Volume: 209; Issue: 1 Linguagem: Inglês

10.1111/j.1574-6968.2002.tb11111.x

ISSN

1574-6968

Autores

Nicolas Sauvageot, Cécile Muller, Axel Hartke, Yanick Auffray, Jean‐Marie Laplace,

Tópico(s)

Amino Acid Enzymes and Metabolism

Resumo

The three genes (pduCDE) encoding the diol dehydratase of Lactobacillus collinoides were sequenced. They exhibited strong identities with the ddrABC and pduCDE genes of Klebsiella oxytoca and Salmonella enterica, respectively. These genes are part of a putative operon with at least four other genes. An eighth open reading frame was identified as homologous to the pocR gene (encoding the operon regulatory protein). Although the enzyme was detected in exponential growth phase, PduCDE activity was increased at the end of exponential phase in presence of 1,2-propanediol.

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