Construction of lacZ promoter probe vectors for use in Synechococcus: application to the identification of CO2-regulated promoters
1990; Elsevier BV; Volume: 90; Issue: 1 Linguagem: Inglês
10.1016/0378-1119(90)90437-v
ISSN1879-0038
AutoresDavid J. Scanlan, Stephen A. Bloye, Nicholas H. Mann, David A. Hodgson, Noel G. Carr,
Tópico(s)Algal biology and biofuel production
ResumoIt was shown that the Escherichia coli lacZ gene could be expressed in the cyanobacterium Synechococcus R2 PCC7942 both as a plasmid-borne form and also integrated into the chromosome. A promoterless form of the lacZ gene was constructed and used as a reporter gene to make transcriptional fusions with cyanobacterial promoters using a shuttle vector system and also via a process of integration by homologous recombination. Synechococcus R2 promoter-lacZ gene fusions were then used to identify CO2-regulated promoters, by quantitatively assessing β-galactosidase activity under high and low CO2 conditions using a fluorescence assay. Several promoters induced under low CO2 conditions were detected.
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