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Actively transcribed genes in the raf oncogene group, located on the X chromosome in mouse and human.

1986; National Academy of Sciences; Volume: 83; Issue: 11 Linguagem: Inglês

10.1073/pnas.83.11.3934

1091-6490

Kay Huebner, A ar-Rushdi, C.A. Griffin, Masaharu Isobe, Christine A. Kozak, Beverly S. Emanuel, L Nagarajan, John L. Cleveland, Tom I. Bonner, M D Goldsborough,

Ubiquitin and proteasome pathways

Murine and human cDNAs, related to but distinct from c-raf-1, have been isolated and designated mA-raf and hA-raf, respectively. The mA-raf and hA-raf cDNAs detect the same murine and human fragments in Southern blots of restriction enzyme-cleaved murine and human cellular DNA. The murine restriction enzyme fragments homologous to mA-raf cDNA cosegregate with mouse chromosome X in a panel of Chinese hamster-mouse hybrid cells, thus localizing the mA-raf locus to mouse chromosome X. Two independently segregating loci, detected by the hA-raf cDNA (or mA-raf cDNA), hA-raf-1 and hA-raf-2, are located on human chromosomes X and 7, respectively. The mA-raf locus and the hA-raf-1 locus are actively transcribed in several mouse and human cell lines.