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Externalization of transferrin receptor in established human cell lines

1987; Academic Press; Volume: 11; Issue: 12 Linguagem: Inglês

10.1016/0309-1651(87)90121-4

1878-240X

Yutaka Kongo, Y Niitsu, Takuji Nishisato, Junji Kato, K. Sasaki, N Tsushima, Michiaki Hirayama, Hitoshi Kondo, I Urushizaki,

RNA Interference and Gene Delivery

The externalization of transferrin receptors was found in established human tumor cell lines at the rate of 10-35 ng/hour/10(6) cells, when they were incubated with transferrin at 37 degrees C. This externalization is inhibited by lowering the incubation temperature to 4 degrees C or eliminating the ligand from the culture medium. Metabolic inhibitors such as sodium azide, colchicine, cytochalasin B and chloroquine also decreased the rate of externalization. Almost 95% of released transferrin receptors were precipitated by centrifugation at 100,000 x g for 30 min, suggesting that transferrin receptor is externalized into the medium as a vesicular form.