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Oligomerization of activated d - and l -guanosine mononucleotides on templates containing d - and l -deoxycytidylate residues

1998; National Academy of Sciences; Volume: 95; Issue: 23 Linguagem: Inglês

10.1073/pnas.95.23.13448

1091-6490

Kozlov Ia, Stefan Pitsch, Leslie E. Orgel,

Carbohydrate Chemistry and Synthesis

The oligomerization of activated d - and l - and racemic guanosine-5′-phosphoro-2-methylimidazole on short templates containing d - and l -deoxycytidylate has been studied. Results obtained with d -oligo(dC)s as templates are similar to those previously reported for experiments with a poly(C) template. When one l -dC or two consecutive l -dCs are introduced into a d -template, regiospecific synthesis of 3′-5′ oligo(G)s proceeds to the end of the template, but three consecutive l -dCs block synthesis. Alternating d -, l -oligomers do not facilitate oligomerization of the d -, l -, and racemic 2-guanosine-5′-phosphoro-2-methylimidazole. We suggest that once a “predominately d -metabolism” existed, occasional l -residues in a template would not have led to the termination of self-replication.