The electrochemiluminescence of ruthenium complex/tripropylamine systems at DNA-modified gold electrodes
2010; Elsevier BV; Volume: 26; Issue: 5 Linguagem: Inglês
10.1016/j.bios.2010.08.074
ISSN1873-4235
AutoresDongyuan Liu, You-Ying Xin, Xiwen He, Xue‐Bo Yin,
Tópico(s)Electrochemical Analysis and Applications
ResumoThe electrochemiluminescence (ECL) behavior of ruthenium complex/tripropylamine (TPA) systems at DNA-modified gold electrode was studied to understand the possible mechanism and to develop new detection platforms. DNA strand, especially double-stranded DNA (ds-DNA), can preconcentrate TPA and acts as the acceptor of the protons released from TPAH+, therefore the improved ECL emission and the low potential ECL were observed. The intercalation of Ru(phen)32+ into ds-DNA was confirmed to be a sensitive and label-free DNA-related detection platform. The above results were validated by the analysis of lysozyme using anti-lysozyme aptamer-modified electrode. This work opens a new field by the use of DNA-modified electrode to develop novel sensing platforms, such as low potential ECL biosensors and Ru(phen)32+ intercalation-based ECL biosensors.
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