DNA Deaminating Ability and Genotoxicity of Nitric Oxide and its Progenitors
1991; American Association for the Advancement of Science; Volume: 254; Issue: 5034 Linguagem: Inglês
10.1126/science.1948068
ISSN1095-9203
AutoresDavid A. Wink, Kazimierz S. Kasprzak, Chris M. Maragos, Rosalie K. Elespuru, Manoj Misra, Tambra M. Dunams, Thomas A. Cebula, Walter Koch, A. W. Andrews, Jane S. Allen, Larry K. Keefer,
Tópico(s)Amino Acid Enzymes and Metabolism
ResumoNitric oxide (NO), a multifaceted bioregulatory agent and an environmental pollutant, can also cause genomic alterations. In vitro, NO deaminated deoxynucleosides, deoxynucleotides, and intact DNA at physiological pH. That similar DNA damage can also occur in vivo was tested by treating Salmonella typhimurium strain TA1535 with three NO-releasing compounds, including nitroglycerin. All proved mutagenic. Observed DNA sequence changes were greater than 99% C----T transitions in the hisG46 (CCC) target codon, consistent with a cytosine-deamination mechanism. Because exposure to endogenously and exogenously produced NO is extensive, this mechanism may contribute to the incidence of deamination-related genetic disease and cancer.
Referência(s)