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Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture

1999; Wiley; Volume: 44; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-4636(19990315)44

1097-4636

Chang Du, F.Z. Cui, Xue-Yang Zhu, K. de Groot,

Electrospun Nanofibers in Biomedical Applications

Journal of Biomedical Materials ResearchVolume 44, Issue 4 p. 407-415 Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture C. Du, C. Du Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. ChinaSearch for more papers by this authorF. Z. Cui, Corresponding Author F. Z. Cui [email protected] Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. ChinaDepartment of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. ChinaSearch for more papers by this authorX. D. Zhu, X. D. Zhu China–Japan Friendship Hospital, Beijing Medical University, Beijing 100038, P.R. ChinaSearch for more papers by this authorK. de Groot, K. de Groot Biomaterials Research Group, Leiden University, Prof. Bronkhorstlaan 10, Building 57, 3723 MB Bilthoven, The NetherlandsSearch for more papers by this author C. Du, C. Du Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. ChinaSearch for more papers by this authorF. Z. Cui, Corresponding Author F. Z. Cui [email protected] Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. ChinaDepartment of Materials Science and Engineering, Tsinghua University, Beijing 100084, P.R. ChinaSearch for more papers by this authorX. D. Zhu, X. D. Zhu China–Japan Friendship Hospital, Beijing Medical University, Beijing 100038, P.R. ChinaSearch for more papers by this authorK. de Groot, K. de Groot Biomaterials Research Group, Leiden University, Prof. Bronkhorstlaan 10, Building 57, 3723 MB Bilthoven, The NetherlandsSearch for more papers by this author First published: 29 January 1999 https://doi.org/10.1002/(SICI)1097-4636(19990315)44:4 3.0.CO;2-TCitations: 283AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Transplantation of osteogenic cells with a suitable matrix is one strategy for engineering bone tissue. Three-dimensional distribution and growth of cells within the porous scaffold are of clinical significance for the repair of large bony defects. A nano-HAp/collagen (nHAC) composite that mimics the natural bone both in composition and microstructure to some extent was employed as a matrix for the tissue engineering of bone. A porous nHAC composite was produced in sheet form and convolved to be a three-dimensional scaffold. Using organ culture techniques and the convolving method, we have developed three-dimensional osteogenic cells/nHAC constructs in vitro. Scanning electron microscopic and histological examination has demonstrated the development of the cells/material complex. Spindle-shaped cells migrating out of bone fragments continuously proliferated and migrated throughout the network of the coil. The porous nHAC scaffold provided a microenvironment resembling that seen in vivo, and cells within the composite eventually acquired a tridimensional polygonal shape. In addition, new bone matrix was synthesized at the interface of bone fragments and the composite. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 407–415, 1999. Citing Literature Volume44, Issue415 March 1999Pages 407-415 RelatedInformation