Voltar
Artigo Revisado por pares
BIBTEX RIS

Determination of Sepharose-bound protein with Coomassie brilliant blue G-250

1985; Elsevier BV; Volume: 151; Issue: 2 Linguagem: Inglês

10.1016/0003-2697(85)90222-2

ISSN

1096-0309

Autores

R.A. Asryants, I.V. Duszenkova, N.K. Nagradova,

Tópico(s)

Advanced Proteomics Techniques and Applications

Resumo

The colorimetric procedure of Bradford (M. M. Bradford, 1976, Anal. Biochem.72, 248–254) was found to be convenient for determining the content of a protein immobilized on Sepharose. Being simple, sensitive, and rapid, this method appears very useful in studies involving multiple analyses of immobilized protein species present at low concentrations.

Referência(s)