Regeneration of Plants from Protoplasts of Arabidopsis thaliana L. cv. Columbia (C24), Via Direct Embryogenesis
1993; Oxford University Press; Volume: 44; Issue: 10 Linguagem: Inglês
10.1093/jxb/44.10.1579
ISSN1460-2431
AutoresCarmel M. O’Neill, R. J. Mathias,
Tópico(s)Plant tissue culture and regeneration
ResumoThis report describes a protocol for the regeneration of fertile plants from mesophyll protoplasts of Arabidopsis Arabidopsis thaliana race Columbia (C24). Regeneration was rapid and reproducible. The protocol is especially novel in that a large proportion of regenerating protoplasts regenerated via direct somatic embryogenesis. Protoplasts isolated from in vitro-grown plants entered sustained division after 3-5 d in culture medium and over a period of several days 6-22% of protoplasts underwent at least one cell division. Approximately 2-16% of these protoplasts continued to divide and after 3 weeks in culture had formed macroscopic colonies, of which 70-80% were regular embryo-like structures
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