Portal de Periódicos da CAPES

A role for the transcription factor Helios in human CD4+CD25+ regulatory T cells

2010; Elsevier BV; Volume: 47; Issue: 7-8 Linguagem: Inglês

10.1016/j.molimm.2010.02.001

1872-9142

Derese Getnet, Joseph F. Grosso, Monica V. Goldberg, Timothy Harris, Hung‐Rong Yen, Tullia C. Bruno, Nicholas M. Durham, Edward L. Hipkiss, Kristin J. Pyle, Satoshi Wada,

CAR-T cell therapy research

Relative upregulation of the Ikaros family transcription factor Helios in natural regulatory T cells (Tregs) has been reported by several groups. However, a role for Helios in regulatory T cells has not yet been described. Here, we show that Helios is upregulated in CD4+CD25+ regulatory T cells. Chromatin-immunoprecipitation (ChIP) experiments indicated that Helios binds to the FoxP3 promoter. These data were further corroborated by experiments showing that knocking-down Helios with siRNA oligonucleotides results in down-regulation of FoxP3. Functionally, we found that suppression of Helios message in CD4+CD25+ T cells significantly attenuates their suppressive function. Taken together, these data suggest that Helios may play an important role in regulatory T cell function and support the concept that Helios may be a novel target to manipulate Treg activity in a clinical setting.