Identification of 9α-fluorinated homologues of hydrocortisone and prednisolone by thin-layer chromatography on insoluble polyvinyl-pyrrolidone
1970; Elsevier BV; Volume: 48; Linguagem: Inglês
10.1016/s0021-9673(01)85537-0
ISSN1873-3778
AutoresJoaquín Moreno Dalmau, Jose María Pla Delfina, Alfonso del Pozo Ojeda,
Tópico(s)Analytical Methods in Pharmaceuticals
ResumoTLC on insoluble crosslinked polyvinyl-pyrrolidone and with dilute HCl solution as the developing solvent has been used successfully to separate the four related synthetic anti-inflammatory corticosteroid, hydrocortisone, prednisolone, 9α-fluorohydrocortisone and 9α-fluoroprednisolone, which differ in one fluorine atom and/or one double bond in position 1. The method is applicable to the identification of the fluorinated analogues in the presence of their respective unfluorinated alcohols merely by comparing their relative migration characteristics. The experimental ΔRM values found for the 9αF atom are around +0.60.
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