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Establishment from an adult leukemic patient of two novel precursor B cell lines with different growth modality

1990; Elsevier BV; Volume: 14; Issue: 2 Linguagem: Inglês

10.1016/0145-2126(90)90047-d

1873-5835

Corrado Tarella, Patrizia Pregno, Eugenio Gallo, Daniele Caracciolo, Silvia De Stefani, C Barletta, Luisa Lanfrancone, Dario Ferrero,

T-cell and Retrovirus Studies

Two novel cell lines, PC-53 and PC-53A were established from an adult ALL patient, at third relapse and in the terminal accelerated phase respectively. Both lines displayed the phenotype of B-cell precursors (CD19+, CD38+, CD20−, cytoplasmic-μ−, immunoglobulin gene rearrangement), identical to the freshly isolated blast cells. Chromosomal analysis showed a prominent 45-XX karyotype, including three marker chromosomes. No chromosome 8 abnormalities were detectable, consistently with a non-rearranged c-myc locus. Both cell lines were EBV-negative. Growth stimulation by autologous supernatant was observed for PC-53 cells during the first 4 months in culture, whereas it was much less evident for PC-53A cells. Thus, PC-53 and PC-53A cells represent a useful tool to investigate the mechanisms involved in the clonal expansion of B-cell precursors.