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Human Immunodeficiency Virus Type 1 Vpr Interacts with Antiapoptotic Mitochondrial Protein HAX-1

2005; American Society for Microbiology; Volume: 79; Issue: 21 Linguagem: Inglês

10.1128/jvi.79.21.13735-13746.2005

1098-5514

Venkat S. R. K. Yedavalli, Hsiu‐Ming Shih, Yu-Ping Chiang, Chun‐Yi Lu, Luan‐Yin Chang, Mao-Yuan Chen, Che‐Yen Chuang, Andrew I. Dayton, Kuan‐Teh Jeang, Li‐Min Huang,

Diabetes and associated disorders

ABSTRACT Human immunodeficiency virus type 1 viral protein R (Vpr) is required for viral pathogenesis and has been implicated in T-cell apoptosis through its activation of caspase 3 and caspase 9 and perturbation of mitochondrial membrane potential. To understand better Vpr-mitochondria interaction, we report here the identification of antiapoptotic mitochondrial protein HAX-1 as a novel Vpr target. We show that Vpr and HAX-1 physically associate with each other. Overexpression of Vpr in cells dislocates HAX-1 from its normal residence in mitochondria and creates mitochondrion instability and cell death. Conversely, overexpression of HAX-1 suppressed the proapoptotic activity of Vpr.