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PATTERNS OF MESSENGER RNA EXPRESSION FOR alpha 1-ADRENOCEPTOR SUBTYPES IN HUMAN CORPUS CAVERNOSUM

1998; Lippincott Williams & Wilkins; Volume: 160; Issue: 2 Linguagem: Inglês

10.1016/s0022-5347(01)62965-3

1527-3792

Jean‐Pierre Dausse, A Leriche, F. Yablonsky,

Hormonal Regulation and Hypertension

No AccessJournal of UrologyInvestigative Urology1 Aug 1998PATTERNS OF MESSENGER RNA EXPRESSION FOR alpha1-ADRENOCEPTOR SUBTYPES IN HUMAN CORPUS CAVERNOSUM JEAN-PIERRE DAUSSE, ALBERT LERICHE, and FRANCOISE YABLONSKY JEAN-PIERRE DAUSSEJEAN-PIERRE DAUSSE More articles by this author , ALBERT LERICHEALBERT LERICHE More articles by this author , and FRANCOISE YABLONSKYFRANCOISE YABLONSKY (YABLONSKY) Requests for reprints: Laboratoires Debat, 153 rue de Buzenval, 92380 Garches, France. More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)62965-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: The present study evaluated the expression of alpha1-adrenoceptor subtypes in human corpus cavernosum. Materials and Methods: The mRNA encoding alpha1a, and alpha1b and alpha1d subtypes were assessed by RNA-directed complementary cDNA synthesis followed by Taq DNA amplification. The level of alpha1 mRNA was calculated in arbitrary optical density units and normalized with respect to the length of the respective cDNA fragments. Results: We found that alpha1a, alpha1b and alpha1d adrenoceptor subtypes are expressed in human corpus cavernosum, with a predominant expression of the alpha1a subtype. Conclusion: These results suggest that alpha1a-adrenoceptor subtype is important and that understanding the biochemical and functional characteristics of this subtype may lead to the development of specific antagonists in the treatment of impotence. References 1 : Physiology of erection and pharmacological management of impotence. J. Urol.1987; 137: 829. Link, Google Scholar 2 : Cholinergic neurotransmission in human corpus cavernosum. I. Responses of isolated tissue. Am. J. 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Volume 160Issue 2August 1998Page: 597-600 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information JEAN-PIERRE DAUSSE More articles by this author ALBERT LERICHE More articles by this author FRANCOISE YABLONSKY (YABLONSKY) Requests for reprints: Laboratoires Debat, 153 rue de Buzenval, 92380 Garches, France. More articles by this author Expand All Advertisement PDF downloadLoading ...