Some problems in quantitative analysis with concentration-sensitive detectors in high-performance liquid chromatography
1977; Elsevier BV; Volume: 142; Linguagem: Inglês
10.1016/s0021-9673(01)92042-4
ISSN1873-3778
Autores Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoBasic problems that arise in quantitative analyses in high-performance liquid chromatography with concentration-sensitive detectors are discussed. The linear range of a spectroscopic (UV) detector is, among other parameters, a function: (1) of the wavelength and its distance from the maximum of the absorbance, and (2) the bandwidth of the monochromator or filter. Experimental methods are proposed for determining the short- and long-time averaged flow-rates and the reproducibility of the sampling system. The short-time averaged flow-rates were measured with four commercially available types of equipment, and the variations of the results were between ±0.6 and ±1.0%. In routine quantitative analysis a reproducibility of ±1% is a desirable aim. The use of internal standards does not decrease these limits. The results with gradient elution method were worse.
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