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Protective Activity of Hydroxytyrosol Metabolites on Erythrocyte Oxidative-Induced Hemolysis

2013; American Chemical Society; Volume: 61; Issue: 27 Linguagem: Inglês

10.1021/jf4016202

1520-5118

Fátima Paiva‐Martins, Filipe Monteiro-Silva, Vasco Almeida, Mafalda Carvalheira, Cristina Helena dos Reis Serra, José E. Rodríguez‐Borges, João Carlos Fernandes, Luı́s Belo, Alice Santos‐Silva,

Alcohol Consumption and Health Effects

The capacity of important hydroxytyrosol metabolites (homovanillyl alcohol, hydroxytyrosol acetate, homovanillyl alcohol acetate, hydroxytyrosol 3′ and 4′-O-glucuronides, and homovanillyl alcohol 4′-O-glucuronide) to protect red blood cells (RBCs) from oxidative injury induced by the radical initiator 2,2′-azo-bis(2-amidinopropane) dihydrochloride (AAPH) or by the natural radical initiator H2O2 was evaluated. In the presence of AAPH, all compounds showed to protect RBCs from hemolysis in a dose-dependent manner, exccept for the homovanillyl alcohol glucuronide, with the order of activity being at 20 μM hydroxytyrosol > hydroxytyrosol glucuronides = hydroxytyrosol acetate = homovanillyl alcohol = homovanillyl acetate > homovanillyl alcohol glucuronide. At 10 μM, hydroxytyrosol, hydroxytyrosol acetate, and hydroxytyrosol glucuronides still protected hemoglobine from oxidation and from morphological RBC changes. In the presence of H2O2, hydroxytyrosol showed to significantly protect RBCs from oxidative hemolysis in a dose-dependent manner, but the hydroxytyrosol glucuronides showed only a limited protection that was independent of the concentration used.