Portal de Periódicos da CAPES

Assessment of Cellular Proliferation of Sebaceous Neoplasms by AgNOR Counts and Immunohistochemical Demonstrations of PCNA and Ki‐67

1995; Wiley; Volume: 22; Issue: 4 Linguagem: Inglês

10.1111/j.1346-8138.1995.tb03380.x

1346-8138

Shin‐ichi Ansai, Shin Koseki, Yutaka Hozumi, Shigeo Kondô, Kazuo ASO,

Cancer and Skin Lesions

Abstract We assessed cellular proliferation of sebaceous neoplasms by AgNOR counts and the immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA) and Ki‐67, using formalin‐fixed and paraffin‐embedded tissue specimens. We used three categories of sebaceous neoplasms: four cases of sebaceoma, three cases of basal cell carcinoma with sebaceous differentiation (BCSD), and seven cases of sebaceous carcinoma (SC). Significant differences were noted between SC and non‐SC tumors (sebaceoma and BCSD) in AgNOR counts and semi‐quantitative grading of PCNA and Ki‐67 labelling indices (P<0.01). When a cut‐off value of 6 was chosen, the AgNOR value discriminated SC from non‐SC tumors with high specificity and sensitivity. When a cut‐off value of 25% was chosen, PCNA and Ki‐67 labelling indices also discriminated between these tumors. Significant differences were not observed between sebaceoma and BCSD with PCNA and Ki‐67 labelling indices. AgNOR counts of BCSD were a little higher than those of sebaceoma, but the number of cases was too small to perform statistical assessment. We consider AgNOR counts and semi‐quantitative grading of PCNA and Ki‐67 labelling indices to be useful in differentiating SC from BCSD and sebaceoma.