Indirect spectrophotometric determination of BIDA, DISIDA, DTPA and MDP in labelled compounds
1989; Pergamon Press; Volume: 40; Issue: 6 Linguagem: Inglês
10.1016/0883-2889(89)90139-1
ISSN1878-1128
AutoresTjaart N. van der Walt, Paul P. Coetzee, P.J. Fourie,
Tópico(s)Molecular Sensors and Ion Detection
ResumoN-(4-(n-butyl)-acetanilide)iminodiacetic acid (BIDA), N-(2,6-diisopropylacetanilide)iminodiacetic acid (DISIDA), diethylenetriaminepentaacetic acid (DTPA) and methylene diphosphonic acid (MDP) are used in labelling kits. The contents of BIDA, DISIDA or MDP of the 99mTc-labelled compounds can be determined (indirectly) spectrophotometrically with copper, eriochrome cyanine R (ECC) and dodecylethyldimethylammonium bromide (DEDA) in a sodium barbital buffered system at pH 8.5. The calibration curves obey Beer's Law from 0 to 40 μg/25 mL for BIDA and DISIDA, 0 to 60 μg/25 mL for DTPA and 0 to 100 μg/10 mL for MDP.
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