Cytogenetic analysis of F 1 , F 2 and BC 1 plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization
2007; Wiley; Volume: 126; Issue: 4 Linguagem: Inglês
10.1111/j.1439-0523.2007.01370.x
ISSN1439-0523
AutoresWenhui Wei, S. F. Zhang, L. J. Wang, J. Li, Bing Chen, Z. Wang, Lixia Luo, Xiaoping Fang,
Tópico(s)Plant Virus Research Studies
ResumoAbstract By intergeneric sexual hybridization between Sinapis alba and Brassica oleracea , F 1 , F 2 and BC 1 progeny plants were produced. S. alba plants (genome SS, 2n = 24) were pollinated with B. oleracea (genome CC, 2n = 18), and the fertile F 1 plants were pollinated with B. oleracea to obtain BC 1 plants. GISH analysis showed that 10 out of 12 F 1 plants had 12 S. alba chromosomes (one full S chromosome set) and nine B. oleracea chromosomes (one C chromosome sets), representing the expected hybrids. However, two F 1 plants had 12 S chromosomes and 18 C chromosomes (two C chromosome sets), indicating unexpected hybrids. A maximum of three trivalents between C and S chromosomes were identified at metaphase I of semi‐fertile F 1 pollen mother cells (PMCs), which indicates homology and chromosome pairing between these two genomes. The C genome had obviously been doubled in two F 2 plants from selfed semi‐fertile F 1 plants. BC 1 plants consisted of 18 C chromosomes and different numbers of one, five and six additional S chromosomes, respectively. Monosomic alien addition lines developed in the present study can be used for B. oleracea breeding and Sinapis alba gene mapping.
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