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Accumulation of dinucleoside polyphosphates in Saccharomyces cerevisiae under stress conditions. High levels are associated with cell death

1986; Elsevier BV; Volume: 68; Issue: 10-11 Linguagem: Francês

10.1016/s0300-9084(86)80069-4

1638-6183

Mireille Baltzinger, Jean‐Pierre Ebel, Pierre Rémy,

Metal complexes synthesis and properties

Adenosine tetraphosphonucleosides (Ap4X) were measured in Saccharomyces cerevisiae by a coupled phosphodiesterase—luciferase assay. After exposure of the cells to cadmium or to hyperthermic treatment (46°C) a marked increase of the cellular pool from 0.08 μM (base level) to 4 μM or higher was observed. The accumulation of Ap4X to high levels is associated with irreversible processes leading to cell death. Nous avons dosé les dinucléosides tétraphosphates adényliques (Ap4X) dans la levure, Saccharomyces cerevisiae, par la méthode de bioluminescence. L'exposition des cellules au cadmium ou à un traitement thermique brutal augmente considérablement le pool intracellulaire d'un niveau de base de 0,08 μM à 4 μM ou plus. La forte accumulation des Ap4X est associée à des phénomènes irréversibles conduisant à la mort cellulaire.