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Gene Cloning and Properties of the RND‐Type Multidrug Efflux Pumps MexPQ‐OpmE and MexMN‐OprM from Pseudomonas aeruginosa

2005; Wiley; Volume: 49; Issue: 11 Linguagem: Inglês

10.1111/j.1348-0421.2005.tb03696.x

1348-0421

Takehiko Mima, Hiroshi Sekiya, Tohru Mizushima, Teruo Kuroda, Tomofusa Tsuchiya,

Antimicrobial Resistance in Staphylococcus

Abstract We cloned two operons for putative RND‐type multidrug efflux pumps from Pseudomonas aeruginosa by a PCR method. We designated the genes in one operon mexPQ (‐ opmE ) and in another operon mexMN . Introduction of the mexPQ‐opmE into drug hypersensitive cells resulted in elevated MICs of macrolides, fluoroquinolones and some other drugs. Introduction of the mexMN into the hypersensitive cells possessing oprM , but not into cells not possessing oprM , resulted in elevated MICs of chloramphenicol and thiamphenicol. Thus, we conclude that MexPQ‐OpmE and MexMN‐OprM are functional multidrug efflux pumps when expressed in P. aeruginosa .