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Analysis of Enzymatic Hydrolysis of k-casein in Milk Using Diffuse Reflectance of Near-infrared Radiation

1994; Volume: 37; Issue: 6 Linguagem: Inglês

10.13031/2013.28287

2151-0059

Daniel Saputra, F. A. Payne, C.L. Hicks,

Proteins in Food Systems

A technique for monitoring and predicting enzymatic hydrolysis of k-casein in milk using diffuse reflectance of near-infrared radiation (NIR) at the wavelengths of 820, 940, 1250, and 1650 nm was developed and tested. Macropeptide concentration which is traditionally referred to as nonprotein nitrogen (NPN) was used as an indicator of enzymatic reaction. The NPN data were fitted to a first order diffusion model to determine the enzymatic reaction constant. It was found that protein concentration, temperature, and enzyme concentration had a significant effect on the diffusion model kinetic constant, kf.