MicroRNA‐223 regulates FOXO1 expression and cell proliferation
2012; Wiley; Volume: 586; Issue: 7 Linguagem: Inglês
10.1016/j.febslet.2012.02.050
ISSN1873-3468
AutoresLihui Wu, Huihui Li, Cheng Jia, Wei Cheng, Mei Yu, Min Peng, Yuanqing Zhu, Qianlei Zhao, Yi Dong, Kang Shao, Anle Wu, Xing Wu,
Tópico(s)MicroRNA in disease regulation
ResumoIn HCT116 colorectal cancer cells, HeLa cervical cancer cells and HuH-7 hepatoma cells, miR-223 is expressed at a low level. Through infection with lentivirus containing miR-223 precursor, miR-223 [corrected] was overexpressed in all these cells. Interestingly, the expression levels of FOXO1 mRNA and protein, and phosphorylation levels became significantly lower than those of their control. FOXO1 was down-regulated mainly in the cytoplasm, while the nuclear FOXO1 level became relatively high compared to the cytoplasm. As the unphosphorylated active form of FOXO1 increased in the cells, cyclin D1/p21/p27 were up-regulated at either mRNA or protein level. Proliferation of the cells was also greatly inhibited when miR-223 was over-expressed. Therein, our data suggest that miR-223 regulates FOXO1 expression and cell proliferation.
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