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Lack of adiponectin promotes formation of cholesterol gallstones in mice

2010; Elsevier BV; Volume: 399; Issue: 3 Linguagem: Inglês

10.1016/j.bbrc.2010.07.075

1090-2104

Hideharu Ogiyama, Yoshihiro Kamada, Shinichi Kiso, Hiroshi Araki, Takuya Yamada, Tamao Nishihara, Kenji Watabe, Yoshihiro Tochino, Shinji Kihara, Tohru Funahashi, Keishi Kanno, Iichiro Shimomura, Shusaku Tsutsui, Susumu Tazuma, Norio Hayashi,

Drug Transport and Resistance Mechanisms

Plasma adiponectin levels are reduced in obese people, and hypoadiponectinemia is recently reported to associate with cholesterol gallstone formation in human. The aim of this study was to examine the role of adiponectin in gallstone formation using adiponectin knockout mice. We analyzed male knockout and C57BL6J mice fed normal or lithogenic diet for 6 weeks. On lithogenic diet, the prevalence rate of gallstone was significantly greater in knockout mice than C57BL6J mice. The molar percentages of beta and omega-muricholic acid were significantly higher and hepatic sterol 12 alpha-hydroxylase expression (cyp8b1) was significantly lower in knockout mice than C57BL6J mice fed normal diet. The bile apolipoprotein A-I protein levels were decreased in knockout mice. Histological examination showed gallbladder wall thickening and accumulation of glycoprotein in the gallbladder of knockout mice. Gallbladder phospholipase A2-IVA expression was significantly higher in knockout mice than in C57BL6J mice fed lithogenic diet. Our results indicate that lack of adiponectin promotes gallstone formation in mice.