Polarized fluorescence and estimated lifetimes of the DNA bases at room temperature
1979; Elsevier BV; Volume: 61; Issue: 3 Linguagem: Inglês
10.1016/0009-2614(79)87174-2
ISSN1873-4448
Autores Tópico(s)RNA Interference and Gene Delivery
ResumoPolarized fluorescence spectra are presented for thymine, cytosine, adenine and guanine in neutral aqueous solution and in rigid neutral ethylene glycol-water (6:4) at ca. −125°C. For thymine and cytosine at 25°C the polarization is nearly as high as in rigid media. Analysis yields fluorescence lifetime (τf) estimates of ⩽2 ps. This limits the likelihood that emission is from a minor species or via a forbidden transition. The lower room temperature polarization for adenine and guanine indicates τf's of ≈ 10 ps which allows for emission from a fluorescent tautomer or via a slightly forbidden transition.
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