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Bacteriological Studies of Uteri of Clinically Normal Cows of Low Fertility

1958; Elsevier BV; Volume: 41; Issue: 1 Linguagem: Inglês

10.3168/jds.s0022-0302(58)90875-0

1529-9066

H. W. Hawk, C.A. Kiddy, J. B. Wilson, Marcia Esposito, A J Winter,

Milk Quality and Mastitis in Dairy Cows

This study was undertaken to determine if the uteri of clinically normal repeatbreeder cows contained microorganisms at 16 days postbreeding, a stage immediately followed by a high incidence of embryonic death.Sixty-nine repeat-breeders were slaughtered, the uteri removed aseptically, and flushed with 15 ml. of 0.1% tryptone broth.Variou~ media were inoculated with the recovered fluid and incubated in air, in 15% C(L and reduced air, and in an anaerobic jar.Triturated endometrial tissue of 35 animals in the study was similarly cultured.Mieroorg'anisms were recovered from the uteri of eight (11.6%) of the 69 repeat-breeders.A yeast, C(~ndida kr~sei, was isolated from four uteri, an Eseherichia species from one, a Pseudomonas from one, Brucdla a.bortus from one~ and a paracolon from one.Embryos were recovered from 31 of 62 uteri from cows with anatomically normal reproductive tracts; three embryos were recovered from the eight infected uteri.Vaginal mucus samples were obtained from 35 of the animals at from 11 to 13 days postbreedin~' mid at slaughter.One cow had a positive mucus ~,gg'lutination test for Vibro feDrs.Nerum "~g'glutination-lysis tests for leptt~sl)irosis were made on all animals.None was positive.Microbial infection apparently accounted for few ,~f the embryonic deaths in these clinically normal repeat-breeders.Embryonic death is a major cause of infertile services in dairy cows of tow fertility; this is est)eeially true in those animals that show no recognized clinical manifestations for which known therapy is indicated (2,5,13,14).It appears that the majority o~' these deaths in such repeat-breeding cows occurs shortly after the 16th day of gestation (5).The I)resenee of low-grade infectious, for whi(,h (,hara(,teristie pathology is not detected, has frequently been proposed as the cause of early embryo,fie mortality in these cows.Infusion of the uteri with antibiotics at the time of, or immediately prior to, breeding, however, did not lessen the embryoni(' death (I5).But the apparent time of death of the embryo in these cows (16 + days) might be the result of infectious agents entering the uterus after the time of treatment.Contradicting the work of Irlberg ctal.( 15), IAndle.v(.9) has reported eonsideral)le increase in the eoneel)tion rate of repeat-