Simultaneous Determination of Ornidazole and its Main Metabolites in Human Plasma by LC–MS/MS: Application to A Pharmacokinetic Study
2014; Future Science Ltd; Volume: 6; Issue: 18 Linguagem: Inglês
10.4155/bio.14.117
ISSN1757-6199
AutoresJiangbo Du, Zhiyu Ma, Yifan Zhang, Ting Wang, Xiaoyan Chen, Dafang Zhong,
Tópico(s)Infectious Encephalopathies and Encephalitis
ResumoOrnidazole is a 5-nitroimidazole antimicrobial agent used for almost 40 years. A novel LC-MS/MS assay was developed and validated for the simultaneous determination of ornidazole and its main metabolites (M3, M6, M16-1, and M16-2) in human plasma.After extraction from 100 μl of plasma by protein precipitation with acetonitrile, all the analytes were separated on a Capcell PAK MG C18 column (100 × 4.6 mm, 5 μm) within 5.0 min and detected by ESI-MS/MS in the positive mode. The validation results met the acceptance criteria as per the US FDA and EMA guidelines.The validated method was successfully applied to a pharmacokinetic study after oral administration of 1000 mg ornidazole to six healthy Chinese volunteers.
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